lenticrisprv2 blast (Addgene inc)

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Addgene inc lenticrisprv2 blast
Lenticrisprv2 Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+blast/lentiCRISPRv2+blast+(Plasmid+%2398293)/bio_rxiv__64898__2026__10__10__758224-462-2-9
Average 96 stars, based on 497 article reviews

lenticrisprv2 blast - by Bioz Stars, 2026-11

96/100 stars

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Synthesized:

Article Title: Heterochromatin boundaries maintain centromere position, size and number.
Article Snippet: .. For SUV39H1/H2, SETDB1 and DNMT3B deletion in the RPE-Neo4p13 background, guide RNAs (gRNAs) were designed (Supplementary Table 2; synthesized by Merk Life Sciences) and cloned into LentiCRISPRv2-Blast (Addgene, 83480) according to the standard lentiCRISPRv2 cloning protocol79. .. Lentivirus was produced in HEK293T cells in a T25 flask using a plasmid mixture (0.62 μg of pMD2 (Addgene, 12259), 1.87 μg of psPAX2 (Addgene, 12260) and 2.5 μg of pLentiCRSPRv2), transfected with Lipofectamine LTX.

Clone Assay:

Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway
Article Snippet: .. Guide RNAs (gRNAs) were cloned into lentiCRISPRv2 Puro (Addgene # 52961) or lentiCRISPRv2 Blast (Addgene # 83480) according to the standard protocol. .. Sequences targeting Ptgs1 or Ptgs2 were adopted from previous literature19 and listed as follows: sgPtgs1 G C A C A C G G A A G G A A A C A T A G, sg-Ptgs2: G T T G T G C T C C C G A A G C C A G A, and sg-Esr1: G C C T A C G C C G T G C G C G A C A C. To package lentivirus, the lentiviral constructs together with psPAX2 (Addgene #12260), and pMD2.G (Addgene #12259) were transfected into HEK293T cells and supernatant were collected after 48 h. Viral containing supernatants were added to the culture medium of cancer cells for 48 h. Antibiotics were added to the cell culture medium to eliminate the cells not been transfected.

Cloning:

Transduction:

Article Title: Energy Flux Regulates Cell Death Induced by California Serogroup Orthobunyaviruses
Article Snippet: .. BE(2)-C cells were engineered to constitutively express Cas9 via lentiviral transduction of lentiCRISPRv2 blast (Addgene, catalog #98293), which was a gift from Brett Stringer [ ]. ..

Expressing:

Article Title: De novo design of potent CRISPR-Cas13 inhibitors.
Article Snippet: .. The LbuCas13a expression construct was generated by Gibson assembly of synthetic gBlocks (Integrated DNA Technologies) into lentiCRISPRv2-Blast (Addgene, 98293; gift from B. Stringer) between AgeI and MluI restriction sites. .. All liquid phage restriction inhibition experiments were performed in a Tecan Infinite M200 plate reader.

Construct:

Generated:

Plasmid Preparation:

Article Title: CLDN4 regulates cell proliferation in small cell lung cancer cells via SAA1 inhibition.
Article Snippet: Small cell lung cancer (SCLC) is a highly aggressive malignancy characterized by rapid progression and high recurrence rates, making it a refractory cancer.. However, its treatment still relies on a combination of platinumbased chemotherapy and etoposide, and a new effective therapeutic strategy is urgently needed.. Claudin-4 (CLDN4) is a tight junction protein that has been reported to regulate malignant phenotypes in various types of cancer.

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